Demonstration of the GPCR Signaling

BioApplication utilizing Transfluor® technology.

Cells expressing both GFP-βarrestin (right) and the

β2-adrenergic receptor were stained with Hoechst

nuclear dye (left) and a red fluorescent cell

membrane marker (center). Images of all three

fluorescent channels were automatically acquired

on an ArrayScan® HCS Reader and analyzed

using the GPCR Signaling BioApplication. 

Nuclei

(blue overlays) and membranes (red spots) are

identified automatically, and the distribution of the

GFP-βarrestin fusion (yellow and green spots) is

determined. In this example of early activation

the receptor is mostly in small pits co-localized

with the membrane.